Micro Propagation of Several Potted Anthurium Accessions Using Spathe Explants

Kurniawan Budiarto


The introduction of new varieties and production system of anthuriums has faced some problems due to the lowmultiplication rates in conventional vegetative and genotypic alteration problem in reproductive propagation. Sincethen, in vitro propagation technique became important to be investigated. The research was carried out fromSeptember 2006 to August 2007 at the Indonesian Ornamental Crops Research Institute. A complete factorialexperiment was designed to accomplish two chronological in vitro activities. The first step dealt with differentincubation sites, i.e. dark and light conditions for callus induction of three potted anthurium accessions, namelyclone no. SM. 001, cv. Alphine and cv. Bonito. The best callus obtained from the incubation treatment were then,transferred into defined media with different formulations to get free-callus plantlets in the second steps. Theresults showed that more progressive callus development from spathe explant was detected on the media ofmodified Nitsch and Nitsch + 1 mg/l 2,D + 1 mg/l Kin + 1 mg/l BA stored at dark after 60 days incubation. After 45days transferring the callus in the same media on light conditions, torpedo-shaped callus was detected. Completeplanlets were obtained after 75 days reculturing the buds in Nitsch and Nitsch medium containing 2,4 D withvarious BA concentrations. Clone SM. 001 and cv. Bonito showed higher number of visible shoot and rootdevelopments compared to cv. Alphine. In general, better shoot and root developments and higher planlets survivedafter acclimatization were observed on the BA concentrations of 2 and 3 mg/l.


Anthurium andreanum, accessions, spathe explant, planlet, in vitro

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Budiarto, K. and W. Handayati. 2007. In vitro propagation of several ant hurium acc es sions us ing leaf explant s. AGRIVITA. 39 (3) : 261 - 268.

Chen, J., R. J. Henny and D. B. McConnell. 2002. Development of new foliage plant cultivars. Florida Agric. Exp. Sta. J. R- 08541: 1 – 7.

Chand, H., M. N. Pearson and P. H. Lovell . 1999. Rapid vegetative multiplication in Colocasia esculenta (L.) Schott (taro). Plt Cell, Tissue, and Organ Cult. 55: 223 - 226.

Keunhle, A. R. and N. Sugii. 1991. Callus induction and plantlet regeneration in tissue culture of Hawaiian anthurium. HortSci. 26 (7): 919 - 921.

M artin, S. P., C. A. Babisud and T. T. Sekioka . 2001. Micropropagation of white taro (Colocasia esculenta var. esculenta) cv. Pikokea, Pololu, Nipopu, and Haukea. HortSci. 28: 543 – 545.

Martin, K., P. Joseph, Dominic, M. Joseph, and V. J. Phillips. 2002. Direct shoot regeneration from lamina explants of two commercial cut flowers cultivars of Anthurium andreanum Hort. In Vitro Cell. And Dev. Biol. 39 (5) : 24 – 28.

Rosario, T. L. and L. A. Lapitan. 1981. Callus and plantlet formation of Anthurium andreanum Lind. Phil Agric. 64 (2): 197 – 202.

Villalobos, V. M., D. W. M. Leung and T. A. Thorpe. 1984. Light-cytokinin interaction in shoot formation in cultured cotyledon explants of radiate pine. Physiol. Plant. 61: 497 - 504.

Valenzuela, M. G. 1998. In vitro culture of Anthurium andreanum L. ‘Gloria Angara’ (Araceae). The Phil. J. of Crop Sci. 23 : 78.

DOI: http://dx.doi.org/10.31258/jnat.11.1.59-63


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