Hidrolisis Pati Ganyong (Canna edulis) dengan Amilase Bakteri Flavobacterium sp. PTBT I untuk Produksi Bioetanol
Abstract
Bioethanol is an alternative energy of fuels produced from vegetable materials. Vegetable materials that can be used as rawmaterial for bioethanol is ganyong because it contains 22.60 g starch in 100 g ganyong. The production of bioethanol fromstarch material consisted of two steps, hydrolysis and fermentation. One of the steps to increase the value of bioethanolfrom starch of ganyong was hydrolysis process using thermostable amylase enzyme isolated from Flavoacterium sp.PTBT I bacteria was isolated from hot spring of Pancuran Tujuh Baturraden. The aim of this research was to use thermostableamylase to hydrolyze starch of ganyong and glucose produced to result bioethanol. The result of this research showed thatthe optimum condition hydrolysis starch of ganyong was using thermostable amylase acquired at substrate concentrationof 3% (b/v), and incubation time of about 75 minutes. The value of bioethanol increased with time of fermentation, from thefirst to fourth day, which was 0.8361; 2.2379; 5.7590 and 10.5787% (v/v), respectively.
Keywords
Full Text:
PDFReferences
Anindyawati, T. 2009. Prospek enzim dan limbah lignoselulosa untuk produksi bioetanol. BS 44(1): 49–56.
Agu, R.C., Amadife, A.E & Ude, C.M. 1997. Combine heat treatment and acid hydrolysys of cassava grate waste (CGW) Biomass of Ethanol Production. Waste Management 17(1): 91–96.
Alexander, R & Joan, M.G. 1993. Basic Biochemical Methods 2nd ed. New York: A John Willey and Sons.
Judoamidjojo, M., Darwis, A.A & Sa’id, E.G. 1992. Teknologi Fermentasi. Jakarta: Penerbit CV. Rajawali.
Lingga. 1986. Bertanam Ubi-Ubian. Yogyakarta: Penerbit Swadaya
.
Martoharsono, S. 1977. Dasar-Dasar Biokimia. Yogyakarta: Gadjah Mada University Press.
Merina, F & Trihadiningrum, Y. 2011. Produksi Bioetanol dari Eceng Gondok (Eichhornia crassipes) dengan Zymomonas mobilis dan Saccharomyces cerevisiae. Prosiding Seminar Nasional Manajemen Teknologi XIII. Surabaya 5 Februari 2011.
Prihandana, R., Noerwijari, K., Gamawati, P.,Adinurani., Setyaningsih, D., Setiadi, S & Handoko, R. 2007. Bioetanol Ubi kayu Bahan Bakar Masa Depan. Jakarta: Agromedia.
Poedjadi, A. 1994. Dasar-Dasar Biokimia. Jakarta : Universitas Indonesia.
Putri, L.S.E. & Sukandar, D. 2008. Konversi pati ganyong (Canna edulis Ker.) menjadi bioetanol melalui hidrolisis asam dan fermentasi. Biodiversitas 9(2): 112–116.
Rahayu, S. 2004. Karakteristik Biokimiawi Enzim Termostabil Penghidrolisis Kitin. Makalah Pengantar Falsafah Sains. Sekolah Pasca Sarjana (S3) Bogor Institut Pertanian Bogor
Reed, G & Nagodawithana, T.W. 1991. Yeast Technolog, 2nd. New York: Van Nostrand Reinhold.
Roslan, A.M., Yee, P.L., U.K.M., Aziz, S.A & Hassan, M.A. 2011. Production of bioethanol from rice straw using cellulase by local aspergillus sp. Int J Agric 6(2): 188– 193.
Samsuri, M., Gozan, M., Mardias, R., Baiquni, M., Hermansyah, H., Wijanarko, A., Prasetya, B & Nasikin, M. 2007. Pemanfaatan sellulosa bagas untuk produksi bioetanol melalui sakarifikasi dan fermentasi serentak dengan enzim xylanase. Makara Teknologi II(11):17–24.
Schlegel, H.G. 1994. Mikrobiologi U Kimia dan Proses. Jurusan Teknik Kimia Fakultas Teknologi Industri, Surabaya: UPN.
Wulan, P.P.D.K., Dianursanti & Amsal, T. 2009. Pemanfaatan Limbah Pisang untuk Pembuatan Etanol. Makalah Proses Kimia Ramah Lingkungan. Departemen Teknik Gas dan Petrokimia, Fakultas Teknik, Depok : Universitas Indonesia.
Yoshimura, T., Hatakawa, M., Takhashi, F & Kawashima, T. 2012. Study of bio-ethanol production from cellulosic waste (rice straw). J. Technology and Education 19(1):19–22.
Yoswathana N., Phuriphipat, P., Treyawutthiwat, P & Eshtiaghi, M. N. 2010. Bioethanol production from rice straw. Energy Research Journal 1(1): 26–31.
DOI: http://dx.doi.org/10.31258/jnat.15.2.92-98
Refbacks
- There are currently no refbacks.
Copyright (c) 2015 Dian Riana Ningsih, Zusfahair, Amin Fatoni
This work is licensed under a Creative Commons Attribution 4.0 International License.