Interaksi Kapang Patogen Fusarium oxysporum dengan Bakteri Kitinolitik Rizosfer Tanaman Jahe dan Pisang

Rejeki Siti Ferniah, Sri Pujiyanto, Susiana Purwantisari, Supriyadi Supriyadi

Abstract


Fusarium oxysporum is a pathogenic fungi for many plants. The fungi have chitin cell wall that can be degraded by chitinase fromchitinolytic bacteria. Aim of this research is determine how the interaction between the bacteria and F.oxysporum. Bacteria were isolatedfrom plant rizosfere. Chitinolytic activity were measured based on the clear zone around the colony in chitin medium. Bacteria and fungiinteraction were determined by an antagonistic test. This research showed that there were 9 chitinolytic bacteria. J4 and P3 had highchitinolytic index, that are 3 and 3.33, respectively. The two isolates antagonist to F.oxysporum, which the bacteria prevent growth of thefungi. The J4 and P3 are alternative biofungicide for F.oxysporum.


Keywords


chitinolytic bacteria, Fusarium oxysporum f.sp zingiberi, interaction

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References


Chernin, L., Ismailov, Z., Haran, S. & Chet, I. 1995. Chitinolytic Enterobacter agglomerans, antagonistic to fungal plant patogens. Appl. Environ. Microbiol 61: 1720-1726.

Fakamizo, T., Honda, Y., Toyoda, H., Ouchi, S. & Goto, S. 1996. Chitinous component of cell wall of Fusarium oxysporum, its structure deduced from chitosanase digestion.. Biosci Biotech Biochem 60: 1705-1708.

Ferniah, R.S, Pujiyanto, S. & Purwantisari, S. 2004. Potensi bakteri kitinolitik sebagai agen pengendali kapang Fusarium oxysporum penyebab penyakit busuk batang tanaman kentang. Seminar Nasional Hasil Pertanian. Yogyakarta.

Gohel, V., Singh, A., Vimal, M., Ashwini, P. & Chhatpar, H.S. 2005. Bioprospecting and antifungal potential of chitinolytic microorganisms. African Journal of Biotechnology 5(2): 54-72.

Gonsalves, A.K. & Fereira, SA. 1994. Fusarium Primer, http//www. Extento.hawaii.edu/kbase/crop/Type/ fusarium_primer.htm.

Hartantinningsih, Y. 2004. Penapisan bakteri kitinolitik tanah pertanian yang berpotensi sebagai pengendali jamur patogen. Laporan Kerja Praktek. UNDIP, Semarang.

Krishnan, H.B., Kim, K.Y. & Krishnan, A.H. 1999. Expression of Serratia macescens chitinase gene in Sinorhizobium fredii USDA 191 and S. meliloti RCR 201 impedes soybean and alfalfa nodulation. MPMI 12: 48-751.

Mitsutomi, M., Kidoh, H., Tomita, H. & Watanabe, T. 1995. The action of Bacillus circulans WL-12 chitinases on partially N-acetylated chitosan. Biosc.i Biotech. Biochem 59: 529-531.

Okazaki, K., Kawabata, T. Nakano, M. & Hayakawa, S. 1999. Purification and properties of chitinase from Arthrobacter sp. NHB-10. Biosci. Biotech. Biochem 63: 1644-1646.

Mubarik, N.R, Mahagini, I, Anindyaputri, A., Santoso, S. & Rusmana, I. 2010. Chitinolytic Bacteria Isolated from Chili Rhizosphere: Chitinase Characterization and Its Application as Biocontrol for Whitefly (Bemisia tabaci Genn.). American J of Agricultural and Biological Sciences 5(4): 430-435.

Pujiyanto, S. 2002. Isolasi dan karakterisasi bakteri kitinolitik serta kloning shotgun gen kitinase dari Ekosistem Air Hitam, Kalimantan Tengah. Tesis, IPB, Bogor. Ferniah, R.S. dan Purwantisari, S. 2004. Uji Antagonisme bakteri kitinolitik sebagai agen pengendali kapang Alternaria solani penyebab penyakit bercak daun tanaman kentang. Seminar Nasional Hasil Pertanian. Yogyakarta.

Pujiyanto, S., Ferniah, R.S. & Purwantisari, S. 2004. Uji Antagonisme bakteri kitinolitik sebagai agen pengendali kapang Alternaria solani penyebab penyakit bercak daun tanaman kentang. Seminar Nasional Hasil Pertanian. Yogyakarta.

Saito A., Fujii, T., Yoneyama, T., Redenbach, M., Onho, T., Watanabe, T. & Miyashita, K. 1999. High-multiplicity of chitinase genes in Streptomyces coelicolor A3(2). Biosci. Biotech. Biochem 63: 710-718.

Svitil, A.L., Chadhain, S.M., Moore, J.A. & Kirchman, D.L. 1997. Chitin degradation proteins produced by the marine bacterium Vibrio harveyi growing on different forms of chitin. Appl Environ Micobiol 63: 408-413.

Singh, P.P., Shin, Y.C., Park, C.S. & Chung, Y.R. 1999. Biological control of Fusarium wilt of cucumber by chitinolitic bacteria. Phytopathology 89: 92-99.

Ueda, M., Shiro, M. Kawaguchi, T. & Arai, M. 1996. Expression of chitinase III gen of Aeromonas 10S-24 in E. coli. Biosci. Biotech. Biochem 60: 1195-1197.

Wang, S.L., Chiou, S.H. & Chang. W.T. 1997. Production of chitinase from shellfish waste by Pseudomonas aeruginosa K-

Proceed. Nat. Sci. Council ROC 21: 71-78.

Yurnalisa. 2001. Kajian peran aktinomisetes kitinolitik dalam pengendalian jamur patogen Fusarium oxysporum skala laboratorium. Tesis. UGM, Yogyakarta.




DOI: http://dx.doi.org/10.31258/jnat.14.1.56-60

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