Pengaruh Sitokinin Eksogen dan Sukrosa terhadap Produksi Biomassa dan Alkaloid Canthinone di dalam Kultur Suspensi Sel Pasak Bumi (Eurycoma longifolia Jack.)

Luthfi Aziz Mahmud Siregar

Abstract


The effect of addition cytokinins and modification of sucrose concentration on growth and alkaloid canthinoneproduction in cell suspension cultures of Eurycoma longifilia Jack were studied. The additions of cytokines, BAand kinetin, show effect on the production of biomass and alkaloid in cell suspension of E. longifilia Jack. Theoptimum totals of two-alkaloids were obtained on addition 4.44 μM BAP and without kinetin, respectively. Theaddition of 4.44 μM BA (6-benzyladenine) into TAM medium stimulated increased total of 9-hydroxycanthine-6-one,but decreased total of 9-methoxycanthin-6-one. While the addition of 2.32 - 9.29 μM kinetin (6-furfurylaminopurine)into TAM medium decreased total of two alkaloids (from 0.582 mg to 0.461 - 0.257 mg per 25 ml medium). Whensucrose concentration in TAM medium was increased from 3% to 5%, production of biomass would increase from0.374 g to 0.585 g dry weight per 25 ml medium. While total of two-alkaloids increase from 0.328 mg to 0.441 mgper 25 ml medium when concentration of sucrose in TAM medium was increased from 3% to 4% sucrose.

Keywords


canthinone, cell suspension, cytokinin, Eurycoma longifolia Jack, sucrose

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Anderson, L.A., Harris, A. & Phillipson, J.D. 1983. Production of cytotoxic canthin-6-one alkaloids by Ailanthus altissima plant cell cultures. J. Nat. Prod. 46: 374-378.

Ang, H.H., Chan, K.L., Gan, E.K. & Yuen, K.H. 1997. Enhancement of sexual motivation in sexually natïve male mice by Eurycoma longifolia Jack. Int. Journ. Pharmacog. 35(2):144- 146.

Akalezi, C.O., Liu, S., Li, Q.S., Yu, J.T. & Zhong, J.J. 1999. Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng. Process Biochem. 34: 639-642.

Blechert, S., Guenard, D. 1990. Taxus alkaloids. Di dalam: Brossi, A. (ed.). The Alkaloids. Chapter 6. Vol. 39. San Diego: Academic Press.

Bhojwani, S.S. & Razdan, M.K. 1996. Plant Tissue Culture: Theory and Practice, A Revised Edition. Netherlands: Elsevier. Amsterdam.

Chan, K.L., O’Neill, M.J., Phillipson, J.D. & Warhurst, D.C. 1986. Plants as sources of antimalarial drugs. Part 3: Eurycoma longifolia Jack. Planta Med. 52(20): 105-107.

Chan, K.L., Lee, S.P., Sam, T.W. & Han, B.H. 1989. A quassinoids glycoside from rhe roots of Eurycoma longifolia Jack. Phytochemistry 28(10): 2857-2859.

Chan, L.K., Siregar, L.A.M. & Chris, Teo, K.H. 1999. In vitro callus and cell cultures of Eurycoma longifolia Jack as affected by types of explants. Proceedings: Seminar of Medicinal Plants-Quality Herbal Products for Healthy Living. Forest Research Institute of Malaysia. CFFPR series: 101- 107.

Chan, K.L., Choo, C.Y., Abdullah, N.R. & Ismail, Z. 2004. Antiplasmodial studies of Eurycoma longifolia Jack using the lactate dehydrogenase assay of Plasmodium falciparum. J. Ethnopharm. 92 (2-3): 223-227.

Choo, C.Y., Nah, B.K., Ibrahim, P. & Chan, K.L. 2000. Antimicrobial activity of Eurycoma longifolia Jack. Di dalam Rahmani, M., Mahmood, M., Sukari, M.A., Lian, G.E.C., Hin, T.Y.Y. and Ali, D.A.I. (eds.). Interdisciplinary Approaches in Natural Products Research. Proceedings of the 16th National Seminar on Natural Products Department of Chemistry. Universiti Putra Malaysia. Serdang, Malaysia. 219-221.

Crespi-Perellino, N., Guicciardi, A., Malyszko, G.& Minghetti, A. 1986. Biosynthetic relationship between indole alkoloids produced by cell cultures of Ailanthus altissima. J. Nat. Prod. 49: 814-822.

Darise, M., Kohda, H., Mizutani, K. & Tanaka, O. 1982. Eurycomanone and Eurycomanol, Quassinoids from The Roots of Eurycoma longifolia. Phytochemistry 21 (8): 2091-2093.

De-Eknamkul, W. & Ellis, B.E. 1985. Effects of macronutrients on growth and rosmarinic acid formation in cell suspension cultures of Anchusa officinalis. Plant Cell Rep. 4 : 46 - 49. Endress, R. 1994. Plant Cell Biotechnology. Berlin, Heidelberg: Springer-Verlag. pp. 121–242.

Fujita, Y. 1988. Industrial production of shikonin and berberine. Di dalam Bock, G. and Marsh, J. (eds.), Application of Plant Cell and Tissue Culture. CIBA Foundation Symposium, vol. 137. Chichester: Wiley. pp. 228-235.

Grzelak, A. & Janiszowska, W. 2002. Initiation and growth characteristics of suspension cultures of Calendula officinalis cells. Plant Cell, Tissue Org. Cult. 71: 29- 40.

Hayashi, T., Yoshida, K. & Sano, K. 1988. Formation of alkaloidsn suspension-cultured Colchicum autumnale. Phytochemistry 27(5): 1371-1374.

Itokawa, H., Kishi, E., Morita, H., Takeya, K., & Iitaka, Y. 1991. Eurylene, a new squelene-type triterpene from Eurycoma longifolia. Tetrahedron Letters. 32: 1803-1804.

Itokawa, H., Kishi, E., Morita, H. & Takeya, K. 1992. Cytotoxic quassinoids and tirucallane-type triterpenes from tne woods of Eurycoma longifolia. Chem. Pharm. Bull. 40: 1053-1055.

Kanchanapoom, T., Chumsri, P., Sonchai, S., Kasai, R. & Yamasaki, K. 2002. Canthin-6-one alkaloids from callus cultures of Eurycoma longifolia. Nat Medic. 56(2): 55-58.

Karam, N.S. Jawad, F.M., Arikat, N.A. & Shibli, R.A. 2003. Growth and rosmarinic acid accumulation in callus, cell suspension and root cultures of wild Salvia fruticosa. Plant Cell Tissue Org. Cult. 73: 117-121.

Kardono, L.B.S., Angehofer C.K., Tsauri S., Padmawinata K., Pezzuto J.M. & Kinghorn D. 1991. Cytotoxic and antimalarial constituents of the roots of Eurycoma longifolia. Journ. Nat. Prod. 54: 1360-1367.

Ketchum, R.E.B., Gibson, D.M. & Greespan Galo, L. 1995. Media optimization for maximum biomass production in cell cultures of pacific yew. Plant Cell Tissue Org. Cult. 42: 185-193.

Liu, K.C.S., Yang, S.L., Roberts, M.F. & Phillipson, J.D. 1990. Canthin-6-one alkaloids from cell suspension cultures of Bruceae javanica. Phytochemistry 29(1): 141-143.

Liu, K.C.S., Roberts, M.F., Homeyer, B.C., Yang, S.L. & Phillipson, J.D. 1994. The effect of plant growth regulators on the production of canthin-6-one alkaloids by Brucea javanica cell suspension cultures. Phytochemistry, 37 (2): 421-424.

Minghetti, A., Perellino, N.C., Garofano, L., Speroni, E. & Vincieri, F.F. 1996. Production of diterpenoids by Euphorbia calyptrata cell cultures. Phytochemistry 42 (6): 1587-1589.

Morita, H., Kishi, E., Takeya, K., Itokawa, H. & Tanaka, O. 1993. Highly oxygenated qussinoids from Eurycoma longifolia. Phytochemistry 33(3): 691-696.

Morimoto, T., Hara, Y., Kato, Y., Hiratsuka, J., Yoshioka, T., Fujita, Y. & Yamda, Y. 1988. Berberine production by cultured Coptis japonica cells in a one-stage culture using medium with a high Copper concentration. Agric. Biol. Chem. 52 (7): 1835-1836.

Murashige, T. & Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.

Narayanaswamy, S. 1994. Plant Cell and Tissue Culture. New Delhi: Tata McGraw-Hill Publishing Company Limited. Ong, B.T. 1999. Perbandingan kualitatif kandungan kimia dalam hasil In vitro Eurycoma longifolia dengan kandungan kimia dalam pokok induk Eurycoma longifolia. Tesis B Sc. Penang: Universiti Sains Malaysia.

Scragg, A.H., Ashton, S., York, A., Bond, P., Stepan- Sarkissian, G. & Grey, D. 1990. Growth of Catharanthus roseus suspensions for maximum biomass and alkaloid accumulation. Enzyme. Microb. Technol. 12: 292-298.

Siregar, L.A.M., Chan L.K. & Teo, C.K.H. 1998. In vitro callus and root production of Eurycoma longifolia. Dalam: Towards the sustainable use the bioresoueces - Challenges and opportunities for then IMT-GT. Proceedings of the Second IMT-GT UNINET Conference. Penang, 29 -30 August, 1998. 121-123.

Siregar, L.A.M., Chan, L.K., Boey, P.L. 2003. Selection of cell source and the effect of pH and MS macronutrients on biomass production in cell cultures of Tongkat Ali (Eurycoma longifolia Jack). J. Plant Biotech. 5(2): 131-135.

Siregar LAM, Chan LK, & Boey PL (2004) Effect of cell source and pH culture medium on the production of canthin-6-one alkaloids from the cell cultures Tongkat Ali (Eurycoma longifolia Jack). J. Plant Biotech. 6(2): 125-130.

Stafford, A. & Warren, G. 1991. Plant Cell and Tissue Culture. Buckingham: Open University Press.

Tada, H., Yasuda, F., Otani, K., Doteuchi, M., Ishihara, Y. & Shiro, M. 1991. New antiulcer quassinoids from Eurycoma longifolia. Europ. J. Medic. Chem. 26: 345-349.

Zhao, D. Xing, J., Li, M., Lu, D. & Zhao Q. 2001. Optimization of growth and jaceodin production in callus and cell suspension cultures of Saussurea medusa. Plant Cell, Tissue Org. Cult. 67: 227-234.




DOI: http://dx.doi.org/10.31258/jnat.12.2.143-151

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