Isolasi dan Identifikasi Bakteri Asam Laktat Proteolitik dari Susu Kedelai yang Terfermentasi Spontan

Yusmarini Yusmarini, Retno Indrati, Tyas Utami, Yustinus Marsono

Abstract


Lactic acid bacteria is a group of bacteria with proteolytic activities enambling to grow on protein rich substratesuch as soymilk. This research was aim to isolate and identify lactic acid bacteria with have proteolytic activityfrom spontaneous fermented soy milk. Sixteen isolates out of 26 colonies isolated from fermented soymilk arepresumed as lactic acid bacteria. Among these 16 isolates, only 3 of them showed proteolytic activity. These threeisolates were further identify morphologically and only two isolates identified as Lactic Acid Bacteria, namelyR.1.3.2 and R.11.1.2. The ability of these isolates to produce acid and protease were observed. The results showedthat isolate R 1.2.3 higher ability in producing protease.

Keywords


fermented soymilk, isolation and identification, lactic acid bacteria, proteolytic activity

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References


Angeles, A.G. & Marth, E.H. 1971. Growth and activity lactic acid bacteria in soy milk, growth and acids production. J. Milk Food Technol 34: 30.

Bordignon, J.R., Nakahara, K., Yoshihashi, T., & Nikkuni, S. 2004. Hydrolysis of isoflavone and comsumption of oligosaccharides during lactic acid fermentation of soybean milk. JARQ 38: 259- 265.

Dakwa, S., Saiki, E., Dawson, Diako, C., Annan, N.T., & Amoa- Awua, W.K. 2005. Effect of boiling and roasting on the fermentation of soybeans into dawadawa (soy-dawadawa). International Journal of Food Microbiology 104: 69-82

Fung, D.Y.C. 1986. Types of microorganisms. ch.2. Di dalam: Cunningham, F.E. & Cox, N.A. The Microbiology of Poultry Meat Product. New York: Academic Press Inc.

Garabal , J.I ., Alon so , P.R., & Cen teno , J.A. 2007. Characterization of lactic acid bacteria isolated from raw cow’s milk cheeses currently produced in Galicia (NW Spain). Swiss Soc. of Food Sci. and Technol.

Gobbetti, M., Smacchi, E., Fox, P., Stepaniak, L., & Corsetti, A. The Sourdough Microflora, Cellular Location and haracterization of Proteollytic Enzymes in Lactic Acid Bacteria. Academic Press Limited. Halaman 561-569.

Lestari, L.A., Harmayani, E., & Marsono, Y. 2004. Efek hipokolesterolemik yogurt yang disuplementasi probiotik. Agrosains 17: 51- 64.

Nakazawa Y. & Hosono, A. 1992. Function of fermented milk. Challenges for The Health Sciences: 180-184S.

Nisa, F.Z., Marsono, Y., & Harmayani, E. 2006. Efek hipokolesterolemik susu kedelai femetasi steril pada model hewan coba. Agrosains 19: 41-53.

Piraino, P., Zotta, T., Ricciardi, A., McSweeney, P.L.H. & Parente, E. 2008. Acids production, proteolysis, autolytic and inhibitory properties of lactic acid bacteria isolated from pasta filata cheese: A multivariate screening study. Int. Dairy Journal 18: 81-92

Rahayu, E.S. & Margino, S. 1997. Bakteri Asam Laktat : Isolasi dan Identifikasi. Materi Workshop, diselenggarakan oleh PAU Pangan Gizi. Universitas Gadjah Mada. Yogyakarta, 13-14 Juni 1997.

Schlegel, H.G. 1994. Mikrobiologi Umum Ed. Ke 6. Yogyakarta: Gadjah Mada University Press.

Steamer, J.R. 1979. Lactic acid bacteria. Di dalam: de Fuguiredo M.P. & Splittoesser, D.F. Food Microbiology. Public Health and Spoilage aspect. Westport: AVI Pub.

Wood, B.J.B. 1992. The Lactic Acid Bacteria in Health and Deseases. London: Blackie Academic and Professional.




DOI: http://dx.doi.org/10.31258/jnat.12.1.28-33

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