Perubahan Bentuk Planlet Pisang Raja Sereh Hasil Mutasi dengan Ethyl Methane Sulphonate (EMS) Secara In Vitro

Yulmira Yanti, Trimurti Habazar, Mardinus ', Mansyurdin '

Abstract


The shoot of “rajasereh” banana was treated by 0,2% and 0,5% of ethyl methane sulfonate (EMS), for 2 and 4 hoursthourgh in vitro. The results showed that treatment of EMS mutagen would be changed morphologycal characterseither in planlet. In planlet stage was obtained four morphololgycal variations. One of the variant have characterizedthe colour yellowish; the leaves and stem are yellowish: the leaves were small and spiral. The value of coefficientof variant for morfphologycal characters of planlet “raja sereh” banana increased compare to the control. Theextreme value of coefficient of variant in planlet stage was found the times shoots is 84,31%, while control that is8,24%. EMS mutagen could caused several planlets shorter, total of leaves could of shoot are mostly

Keywords


EMS (Ethyl Methane Sulfonate), Mutagen, In Vitro, Morphology “raja sereh” Banana

Full Text:

PDF

References


Bhagwat, B & Duncan, E. J. 1998. Mutation breeding of banana c.v. Highgate, (Musa spp., AAA grup) for tolerance to Fusarium oxysporum f. sp. cubence using chemical mutagen. Musarama 12: 5-15.

EPP, M. D. 1987. Somaclonal Variation in Bananas: a case study with Fusarium wilt, Banana and Plantain Breeding Breeding Strategies (Persley, G.J., De Langhe, E.A., Eds). ACIAR. Canberra 21: 140-150.

Fegan & Prior. 2005. Bacterial Wilt Disease and the “Ralstonia solanacearum species complex” (Eds by C. Allen., P. Prior., A.C. Hayward) St. Paul. USA: APS Press.

Gardiner, E. J. & Peteer, S. 1984. Principles of Genetic. Seventh Edition, New York. Chichester: Brisbhani Toronto Singapore.

Gupta, P.K., Singh, S. P. & Bahl, J. R. 1996. A new mungbean variety through induced mutation. Mutation Breeding Newsletter 42: 6-7.

Hwang, S.C. 1990. Somaclonal resistence of Cavendish banana to Fusarium wilt. Proc. Of Regional Tehnical Meeting on Disease Affecting banana and Plantain in Asia and Pasific, Brisbane. Australia, 15-18 April 1990.

Herawati Tin. 1999. Pemuliaan Tanaman Lanjutan. Program Pengembangan Kemampuan Peneliti Tingkat S1 Non Pemuliaan dalam Ilmu dan Teknologi Pemuliaan. Bandung

Jamaluddin, S.A. 1995. Mutation breeding of banana in Malaysia, Musarama 7: 5-20.

Khairwal, I.S, S. Singh & Paroda. 1984. Indiced mutation in surgance. Surgance 3: 14-16

Micke, A. 1996. 70 years included mutations to be reconsidered. Mutation Breeding Newsletter 42: 22-24.

Novak, F.J. 1990. Mutation induction by gamma irradition of irradition of in vitro cultured shoot-tips of banana and plantain (Musa cvs). Trop. Agr. Trinidad 67: 21-28

Mak, C. 1995. Early screening techniques for Fusarium wilt resistance in banana micropagated plants.

Omar, M. S., F. J. Novak & Brunner. 1989. In vitro action of Ethyl Methane sulfonate on banana shoot tips. Scientia Hortic 40: 283-295

Ortiz & D. R. Vulysteke. 1995. Expression of the dihydroflavonoid reductase gene in anthocyanin-free barley mutant. Hereditas 119: 67-75.

Rukmana, R. 1999. Usaha tani Pisang. Yogyakarta: Penerbit Kanisius.

Rahmi, A. 2003. Induksi mutasi pisang Ambon dengan Senyawa Kimia. Skiripsi Sarjana Biologi FMIPA- Universitas Andalas Padang.

Raisinghani,G. & S.K. Mahna. 1996. Behaviour of two induced chlorophyll mutants of Trigonella cornicullata L. Mutation Breeding Newletter 42:17-18.

Roesmiyanto & Hutagalung. 1989. Penyakit Darah (Pseudomonas celebensis) pada tanaman pisang di Senoponto, Sulawesi Selatan. Holtikultura 27: 39-41.

Roux, N.S. 2004.”Improved methods to increase diversity in Musa using mutation and tissue culture techniques” (Report of second Research Co-ordination Meeting of culture FAO/IAEA/BADC Co-ordinated Research Project) Kuala Lumpur. 49-56.

Sahlan & Nurhadi. 1994. Inventarisasi penyakit pisang di sentra produksi Sumatera Barat, Jawa Barat dan Lampung. Penel. Hort. 6: 36-43.

Satyanaraya, M, Y. Tatacharu & T.B., Dasarady. 1980. Effect of chemichal mutagen on sporouting and survival of banana corm, Musarama 8: 36-64.

Siti Hawa. 1996. The generation of variants of musa CV Rastali (AAB) through in vitro gamma radiation evaluotion for important agronomic traits. Musarama 12: 50-71.

Valmayor, R.V. 1999. Banana and Plantain in the Pilipines In : Banana Plantain R and D in Asia and the Pacific proceeding of a Regional Consultation on Banana and Plantain R and D Networking Manila and Davno 87-120.

Wisnubroto, Syaifulla & Satsijati. 1994. Hasil Penelitian holtikultura Pelita V. Jakarta: Puslitbangtan.




DOI: http://dx.doi.org/10.31258/jnat.11.2.104-108

Refbacks

  • There are currently no refbacks.


Copyright (c) 2012 Yulmira Yanti, Trimurti Habazar, Mardinus ', Mansyurdin '

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.