RESPONSE IMMUNITY OF GOLDFISH (Carassius auratus) INFECTED WITH Aeromonas hydrophila BACTERIA AND POST-TREATMENT WITH PROPOLIS SOLUTION
DOI:
https://doi.org/10.31258/ajoas.8.2.223-229Keywords:
Leukocytes, Phagocytosis, Immunity, Carassius auratus, Aeromonas hydrophilaAbstract
This study aims to determine the immunity response of Carassius auratus infected with Aeromonas hydrophila bacteria and post-treatment with propolis solution by measuring total leukocytes and phagocytosis index. The method used is experimental by applying a completely randomized design (CRD) with five treatments and three replications. The treatments were Kn (not infected with A. hydrophila and not treated with propolis), Kp (infected with A. hydrophila but not treated with propolis), while fish infected with A. hydrophila were treated with propolis doses P1 (700 ppm), P2 (800 ppm), and P3 (900 ppm). Treatment was done by injecting 0.1 ml of propolis into fish infected with A. hydrophila intramuscularly. The test material was 150 fish of 8-10 cm in size. The results showed that propolis significantly gave an immune response to the C. auratus infected with A. hydrophila (p<0.05). The 800 ppm propolis dose was the most effective, as indicated by a total leukocyte count of 3.70×104 cells/mm3, a phagocytosis index value of 28.33%, and a survival rate of 83.33%. This study highlights that propolis has potential as a natural immunostimulatory agent in enhancing non-specific defense mechanisms in C. auratus infected with pathogenic bacteria
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1. Lu, H., Tao, T.L., Liu, Y.L., Jia, F., Cui, Y.F., & Shan, X.F. Comparative Evaluation of Selected Adjuvants for Possible Application of Aeromonas caviae Inactivated Vaccine in Crucian Carp (Carassius auratus). Aquaculture International, 2025; 33(5): 1-20
2. Rashidian, G., Boldaji, J.T., Rainis, S., Prokić, M.D., & Faggio, C. Oregano (Origanum vulgare) Extract Enhances Zebrafish (Danio rerio) Growth Performance, Serum, and Mucus Innate Immune Responses, and Resistance Against Aeromonas hydrophila Challenge. Animals, 2021; 11(2): 299
3. El Alem, M.M., Hamed, T.A., & Mohamed, D.T. Pathological and Biochemical Studies on some Antimicrobials in Clarias gariepinus Fish Infected with Aeromonas hydrophila. Zagazig Veterinary Journal, 2017; 45(2): 143-155.
4. Hassanien, H.A., Alrashada, Y.N., Abbas, A.O., & Abdelwahab, A.M. Dietary Propolis Complementation Relieves the Physiological and Growth Deterioration Induced by Flavobacterium columnare Infection in Juveniles of Common Carp (Cyprinus carpio). Plos one, 2023; 18(10): e0292976.
5. Orsi, R.O., Santos, V.G., Pezzato, L.E., Carvalho, P.L.D., Teixeira, C.P., Freitas, J., ... & Barros, M.M. Activity of Brazilian Propolis Against Aeromonas hydrophila and its Effect on Nile Tilapia Growth, Hematological and Non-Specific Immune Response under Bacterial Infection. Anais da Academia Brasileira de Ciências, 2017; 89: 1785-1799
6. Yonar, M.E., Yonar, S.M., & Silici, S. Protective Effect of Propolis against Oxidative Stress and Immunosuppression Induced by Oxytetracycline in Rainbow Trout (Oncorhynchus mykiss, W.). Fish & Shellfish Immunology, 2011; 31(2): 318-325.
7. Edrees, A., Abdel-Daim, A.S., Shaban, N.S., Shehata, O., & Ibrahim, R.E. Dietary Intervention of Propolis and/or Turmeric Boosted Growth, Hematology, Biochemical Profile, and Antioxidant-Immune Responses and their Associated Gene Expression in Nile tilapia (Oreochromis niloticus) Challenged with Edwardsiella tarda. Aquaculture International, 2025; 33(1): 46
8. Blaxhall, P.C., & Daisley, K.W. Routine Haematological Methods for Use with Fish Blood. Journal of Fish Biology, 1973; 5(6): 771-781.
9. Siwicki, A.K., Anderson, D.P., & Waluga, J. Fish Disease Diagnosis and Prevention Methods. Blackwell Publishing, Inc. 1993.
10. Effendi, H. Telaah Kualitas Air bagi Pengelolaan Sumber Daya dan Lingkungan Perairan. Kanisius. Yogyakarta. 2002; 257pp
11. Kurniawan, R., Putri, M.N., Riswan, M., Wahyuni, S., & Mursawal, A. Immunostimulant Effect of Chaetomorpha sp in Tilapia Infected with Aeromonas hydrophila. Aceh Journal of Animal Science, 2025; 10(2): 64-69
12. Chen, H., & Luo, D. Application of Haematology Parameters for Health Management in Fish Farms. Reviews in Aquaculture, 2023; 15(2): 704-737
13. Pattipeilohy, C.E., Suprayudi, M.A., Setiawati, M., & Ekasari, J. Evaluation of Protein Sparing Effect in Nile tilapia Oreochromis niloticus Fed with Organic Selenium Supplemented Diet. Jurnal Akuakultur Indonesia, 2020; 19(1): 84-94
14. Alishahi, M., Tollabi, M., & Ghorbanpour, M. Comparison of the Adjuvant Effect of Propolis and Freund on the Efficacy of Aeromonas hydrophila Vaccine in Common Carp (Cyprinus carpio). Iranian Journal of Fisheries Sciences, 2019; 18(3): 428-444.
15. Pereira, C., Duarte, J., Costa, P., Braz, M., & Almeida, A. Bacteriophages in the Control of Aeromonas sp. in Aquaculture Systems: An Integrative View. Antibiotics, 2022; 11(2): 163
16. Biller, J.D., & Takahashi, L.S. Oxidative Stress and Fish Immune System: Phagocytosis and Leukocyte Respiratory Burst Activity. Anais da Academia Brasileira de Ciências, 90: 3403-3414.
17. Souza, C.D.F., Baldissera, M.D., Verdi, C.M., Santos, R.C., Da Rocha, M.I.U., da Veiga, M.L., ... & Baldisserotto, B. Oxidative Stress and Antioxidant Responses in Nile tilapia Oreochromis niloticus Experimentally Infected by Providencia rettgeri. Microbial Pathogenesis, 2019; 131: 164-169
18. El-Asely, A.M., Abbass, A.A., & Austin, B. Honey bee Pollen Improves Growth, Immunity, and Protection of Nile tilapia (Oreochromis niloticus) against Infection with Aeromonas hydrophila. Fish & Shellfish Immunology, 2017; 40(2): 500-506
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Copyright (c) 2025 M Riswan, Iesje Lukistyowati, Henni Syawal, Ronal Kurniawan, Rianti Putri, Mega Novia Putri (Author)
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