Pertumbuhan Jaringan Stipe dari Jamur Sagu (Volvariella sp) Endemik Papua dalam Kultur in vitro

Barahima Abbas, Florentina Heningtyas Listyorini, Eko Agus Martanto, Yanuarius Renwarin


Sago mushrooms are edible fungus which it grew naturally on decaying sago pith. The objectives of the research were defined an appropriate formulation medium for growth and development of stipe tissue of sago mushroom in order to result spawn stages. The parent of the tissues used as explants were selected from the best performance of sago mushroom which grew naturally. The research divided into three experiments: 1) Initiation of growth hyphae from stipe tissue; 2) hyphae proliferation; and 3) spawn formation. Murashige and Skoog (MS) and Knudson-C (KN) medium enriched 15 and 30% coconut water are the best medium for growth initiation and proliferation of hyphae. Both of these medium induced the explant grew rapidly and resulted vigorous hyphae. No different effect among concentration treatment of coconut water was observed for initiation and proliferation hyphae. The fact that all of the formulation medium used for spawn formation were able to induce spawn development become spawn stage. Even so, the best mediums for spawn formation were medium formulation 9 and 10.


endemic, in vitro, papua, sago mushroom, Volvariella sp

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Abbas, B, Listyorini, F.H & Martanto 2011. Karakteristik jamur sagu (Volvariella sp.) endemik Papua. Jurnal Natur Indonesia 13(2): 168-173.

Atkin, F.C. 1995. Guide to Mushroom Growing. London: Faber and Faber Queen Aqure.

Beausejour, T.M. 1999. Getting started with mushroom cultivation. A MycoWeb Page, Copyright@Michael Wood. html (5 Juni 2010).

Chang, S.T. 1983. A morfological study of Volvariella volvacea. The Chung Chi Journal 91-103.

Chang, S.T & Hayer, W.A. 1978. The Biology and Cultivation of Edible Mushroom. New York: Acad Press.

Chang, S.T. 1999. Global impact of edibel and medical mushroom on human welfare in the 21st century. Int J

Med Mushroom 1: 1-7.

Cordeiro, M.S., Silva, E.H.S., Miranda, M.S., Biondi, F.C., Santos, S.S.D & Ohashi, O.M. 2006. The used of coconut water solution (Cocos nucifera) as a holding medium for immature bovin oocytes for in vitro embryo production. Anim Reprod 3(3): 376-379.

Dodds, J.H & Roberts, L.W. 1986. Experiment in Plant Tissue Culture. 2nd ed. America: Cambridge University Press.

George, O.L & Sherrington, P.D. 1984. Plant Propagation by Tissue Culture. Hand Book and Directory of Commercial Laboratories. England: Exegetics Ltd.

Istimewa. 2008. Jamur kaya nutrisi dari limbah pertanian. http://www.ilmupedia. com/akademik/ 29/615-jamur- kaya-nutrisi-dari-limbah-pertanian.html (22 Juli 2009).

Jordan, M. 1993. Edibel Mushroom and Other Fungi. London: Blanford.

Khayri, J.M.A., Huang, F.H., Morelock, T.E & Busharat, T.A. 1992. Spinach tissue culture improved with coconut water. Hort Science 27: 357-358.

Kuo, M. 2008. The Genus Volvariella. Retrieved from the mushroom Expert. http://www. volvariella.html (17 April 2009).

Li, G.S.F. 1982. Morfology of Volvariella volvacea. Di dalam Shu-ting Chang, T. H. Quimio (eds) Tropical mushrooms: biological nature and cultivation methods. Pp.117–160. Science. books?isbn=9622012647 (20 Juli 2009).

Minh, T.V. 2001. Application of tissue culture techniques in woody species conservations, improvement and development in Vietnam: Mangosteen (Garcinia mangostana L.) via embryogenesis culture. Physiol plant 15: 473-479.

Mohi, L. 2007. Jamur multi manfaat dan pasar luas. http://ikm.depperin. Publikasi. Promosi/ KumpulanArtikel/tabid/67/articleType/ArticleView/ articleId/25/Jamur-Multi-Manfaat-Pasar-Makin- Luas.aspx (10 April 2009).

Moncalvo, J.M., Vilgalys, R., Redhead, A., Johnson, J.M., James, T.Y.,Aime, M.C., Hofstetter, V., Verduin, S.J.W., Larson, E., Baroni, T.J., Thorn, R.G., Jacobsson, S., Clemencon, H & Miller, O.K. 2002. One hundred and seventeen clades of euagarics. Molecular Phylogenetics and Evolution 23: 357-400.

Mshandete, A.M & Cuff, J. 2008. Cultivation of three types of indigenous wild edible mushroom: Cprinus cinerius, Pleurotus flabellatus, and Volvariella volvocea on composted sisal decorication residue in Tanzania. Afr J Bioteknol 7(24): 4551-4562.

Mukhtar, R., Khan, M.M., Rafiq, R., Shahid,A& Khan, F.A. 2005. In vitro regeneration and somatic embryogenesis in Citrus aurantifolia and Citrus sinensis. Int J Agri Biol 7(3): 518-520.

Muhammed, M & Vladimir, B. 2007. Method of preparation and use of coconut water in mamalian tissue nourishment growth and healthy maintenance.

(16 September 2009).

Osemwegie, O.O., Eriyamremu, G.E &Abdulmalik, J. 2006. A survey of macrofungi in Edo/Deltaregion of Nigeria, their morfology and uses. GJPAS 12(2): 149-157.

Pierik, R.L.M. 1987. In Vitro Culture of Higher Plants Martinus. Netherlands: Higoff Publ.

Piexe, P., Raposo, A., Lourenco, R., Cardoso, H & Macedo, E. 2007. Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation. Scientia Horticulturae 113: 1-7.

Roy, B & Mandal, A.B. 2005. Anther culture response in Indica rice and variations in major agronomic characters among the androclones of a scented cultivar, Karnal local. Afr J Biotechnol 4(3): 235-240.

Santoso, H.B. 2003. Air Kelapa, Limbah Penuh Khasiat. h t t p : / / w w w . g i z i . n e t / c g i - b i n / b e r i t a fullnews.cgi?newsid1068615913,84324, (3 September 2009).

Syafii, W,. Bey, Y & Gusmarini. 2000. Pengaruh air kelapa terhadap pertumbuhan tunas melon (Cucumis melo var. Honey dew) pada kultur jaringan. Jurnal Natur Indonesia 2(2): 132-137.

Widiyastuti, B. 2005. Budidaya Jamur Kompos. Jamur merang dan Jamur Kancing. Jakarta: Penebar Swadaya.



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