Utilization of Sago Liquid Waste Organic Fertilizer as a Culture Medium for Chlorella sp.

Irwan Effendi, Mega Novia Putri, Ulfi Laili Astika, Ronal Kurniawan

Abstract


Chlorella sp. is a microalga that can grow and develop in wastewater media, including sago liquid waste that is not utilized and becomes a pollution material. This study aims to determine the utilization of sago liquid waste organic fertilizer on the growth of Chlorella sp. This research was conducted in February 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Riau University. The method used was the experimental method, by applying a complete randomized design (CRD) with four treatments and three replicates. The treatments were as follows: T0 (control), T1 (150 ml sago liquid waste), T2 (200 ml), and T3 (250 ml). The initial density of Chlorella sp. was 250 x103 cells/ml, and cell abundance observations were made for 14 days. The parameters observed were cell abundance, specific growth rate, and water quality (temperature and pH). The results showed that the utilization of sago liquid waste affected the abundance of Chlorella sp. 150 ml concentration and gave the best results on cell abundance of 233.33 x103 cells/ml with the peak population occurring on day 9. Water quality during the study was still in the normal range and can be tolerated for the growth of Chlorella sp, namely temperature ranging from 26-31o C and pH 6.2-8.0

Keywords


Liquid Waste; Cell Abundance; Microalgae

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References


Abd El-Hack, M.E., Abdelnour, S., Alagawany, M., Abdo, M., Sakr, M.A., Khafaga, A.F., Mahgoub, S.A., Elnesr, S.S., Gebriel, M.G., 2019. Microalgae in Modern Cancer Therapy: Current Knowledge. Biomed. Pharmacother, 111: 42–50.

Alagawany, M., Taha, A.E., Noreldin, A., El-Tarabily, K.A., Abd El-Hack M.E., 2021. Nutritional Applications of Spesies of Spirulina and Chlorella in Farmed Fish: A Review. Aquaculture, 542; 736841

Armanda, D.T., 2013. Pertumbuhan Kultur Mikroalga Diatom Skeletonema costatum (Greville) cleve Isolat Jepara pada Medium f/2 dan Medium Conway. Bioma. 2 (1): 49-63.

Balaira, G., Kemer, K., Mantiri, D.M.H., 2017. Pemisahan Pigmen pada Mikroalga Dunaliella salina yang telah Diberi Senyawa Timbal Asetat. Jurnal Pesisir dan Laut Tropis, 5(1); 41-49.

Biolita, N.O., Harmadi., 2017. Perancangan Fotobioreaktor Mikroalga Chlorella vulgaris untuk Mengoptimalkan Konsentrasi Oksigen (O2). Jurnal Fisika Unand, 6 (3): 296-305.

Boroh, R., Litaay, M., Umar, M.R., Ambeng., 2019. Pertumbuhan Chlorella sp. pada Beberapa Kombinasi Media Kultur. Jurnal Biologi Makasar. 4(2) : 129-137

Buwono, N.R., Mahmudi, M., Sabtaningsih, S.O., Lusiana, E., 2019. Analysis of Natural Feed Digestion in Koi Fish Larvae. The Indonesian Green Technology Journal, 8(1): 11-16. https://doi.org/10.21776/ub.igtj.2019.008.01.03

Canelli, G., Tarnutzer, C., Carpine, R., Neutsch, L., Bolten, C.J., Dionisi, F., Mathys, A., 2020. Biochemical and Nutritional Evaluation of Chlorella and Auxenochlorella Biomasses Relevant for Food Application. Frontier in Nutrition, 7:565996.

Dahril, T., Mulyadi, A., Eddiwan., 2020. A Prospect to Develop Chlorella sp. Industry in Riau Province, Indonesia. IOP Conference Series: Earth and Environmental Science, 460; 012042. https://doi.org/10.1088/1755-1315/460/1/012042.

Darosman, T.C., Muhammadar, A.A., Satria, S., 2019. Effect of Rotifera Enrichment (Brachionus plicatilis) with Chlorella sp. for Seabass Larvae (Lates calcarifer). Jurnal Ilmiah Mahasiswa Kelautan dan Perikanan Unsyiah, 4(2): 124-133

Dianita, I., Hasibuan, S., Syafriadiman., 2020. The Effect of Phaseolus radiates Fertilizer at Media Culture to Density and Carotenoid Content of Dunaliella salina. Jurnal Perikanan dan Kelautan, 25(1): 18–26. http://dx.doi.org/10.31258/jpk.25.1.18-26

Fernandiaz, R., Harahap, S., Budijono., 2017. The Effectiveness of Sago Liquid Waste as Nutrient of Chlorella sp. Culture in the Peat Water Media. Jurnal Online Mahasiswa Fakultas Perikanan dan Ilmu Kelautan, Universitas Riau, 4(1): 1-9

Ginting, A.N., Hasan, U., Siswoyo, B.H., 2022. Pemanfaatan Kotoran Burung Puyuh dan Pupuk Organik Cair Azolla pinnata terhadap Populasi serta Kepadatan Sel Spirulina sp. Jurnal Aquaculture Indonesia, 1(2), 120–130.

Hastuti, D.S., Handajani, H., 2001. Budidaya Pakan Alami. Fakultas Peternakan-Perikanan UMM. Malang.

Hendra, N., 2019. Pengaruh Perbedaan Fotopriode pada Kultur Chlorella sp. dengan Sistem Fotobioreaktor Kontinu. Skripsi. Fakultas Perikanan dan Kelautan. Universitas Riau, Pekanbaru. 24 p.

Indriana, N., Idris, M., Ruslaini., Abidin, L.O.B., Aslan, L.O.M., 2020. Pengaruh Konsentrasi Pupuk Organik Cair Lemna (Lemna minor) yang Berbeda terhadap Pertumbuhan Mikroalga Chlorella vulgaris. Jurnal Ilmiah Jurusan Budidaya Perairan, 5(1): 1-12

Jeheskiel, A.V., Kemer, K., Mantiri, D.M.H., Paulus, J., Rompas, R.M., Khreekhoff, R., 2022. Effect of Lead Acetat (Pb(CH3COO)2 on the Growth of Marine Microalgaes Chlorella vulgaris (Beijerink, 1890). Jurnal Ilmiah Platax, 10(2): 363-370.

Lestari, S., 2013. Profil Pertumbuhan dan Analisis Kandungan Karbohidrat, Protein dan Lipid Mikroalga Hijau-Biru pada Medium Af-6 dengan Penambahan Substrat Limbah Ampas Sagu. Universitas Pendidikan Indonesia. Bandung.

Mahdi, M.Z., Titisari, Y.N., Hadiyanto, H., 2012. Evaluasi Pertumbuhan Mikroalga dalam Medium Pome: Variasi Jenis Mikroalga, Medium, dan Waktu Penambahan Nutrient. Jurnal Teknologi Kimia dan Industri, 1(1): 312-319

Meria, R., Widya, P., Ilham Z., 2021. Teknik Kultur Nannochloropsis sp. Skala Laboratorium di Balai Perikanan Budidaya Air Payau Ujung Batee, Aceh Besar. Kenanga (Journal of Biological and Applied Biology), 1(1): 1–9.

Meritasari, D., Mubarok, A.S., Sulmartiwi, L., Masithah, E.D., 2012. Pengaruh Pemberian Pupuk Cair Limbah Ikan Lemuru (Sardinella sp.) dengan Dosis yang Berbeda terhadap Pertumbuhan Chlorella sp. Jurnal Ilmiah Perikanan dan Kelautan, 1(4)

Mookiah, V.P., Kasimani, R., Pandian, S., Asokan, T., 2020. Study on the Effects of Initial pH, Temperature, and Agitation Speed on Lipid Production by Yarrowia lipolytica and Chlorella vulgaris using Sago Wastewater as a Substrate. Tierärztliche Praxis, 40: 692-703

Mufidah, A., Agustono., Sudarno., Nindarwi, D.D., 2017. Teknik Kultur Chlorella sp. Skala Laboratorium dan Intermediet di Balai Perikanan Budidaya Air Payau (BPBAP) Situbondo Jawa Timur. Journal of Aquaculture and Fish Health. 7(2) : 50-56.

Munir, F., Hariyati, R., Wiryani, E., 2017. Pengaruh Limbah Cair Tahu terhadap Pertumbuhan Chick dalam Skala Laboratorium. Jurnal Biologi, 6(2): 84-92.

Novianti, T., Zainuri, M., Widowati, I., 2017. Studi tentang Pertumbuhan Mikroalga Chlorella vulgaris yang Dikultivasi berdasarkan Sumber Cahaya yang Berbeda. Mangifera Edu: Jurnal Biologi dan Pendidikan Biologi, 1(2): 1-8

Nugraha, S., Ekasari, J., Junior, M.Z., Widanarni., 2020. Digestive System and Growth Performance of African Catfish Larvae Clarias gariepinus, (Burchell, 1822) Maintained with Biofloc Technology with the Addition of Chlorella sp. Jurnal Ikhtiologi Indonesia, 20(3): 297-306.

Nuraini., Sukendi., Simanjuntak, L.M., 2020. The Effect of use of Liquid Organic Fertilizer of Turi Putih (Sesbania grandiflora) Leaves with Different Doses of Population Density and Growth Rate of Chlorella sp. Berkala Perikanan Terubuk, 48(3): 1-8

Priyadi, A., Kusrini, E., Megawati, T., 2010. Perlakuan Berbagai Jenis Pakan Alami untuk Meningkatkan Pertumbuhan dan Sintasan Larva Ikan Upside Down Catfish (Synodontis nigriventris). Prosiding Forum Inovasi Teknologi Akuakultur, 2010. Jakarta. 749-754 p.

Putra, S.K., Marten, T.W., Yuniarti, E., Handayuni, L., 2023. Potential of Chlorella sp as a Bioremediation Agent in Tofu Liquid Waste: Literature Review. Jurnal Biologi Tropis, 23(1): 140-146.

Restuhadi, F., Zalfiatri, Y., Pringgondani, D.A., 2017. Symbiotic Mutualism of Starbact and Microalgae Chlorella sp. in Reducing Pollution from Sago Milling Effluent. Jurnal Imu Lingkungan, 11(2): 140-153

Rini, I.S., 2012. Pengaruh Konsentrasi Limbah Cair Tahu terhadap Pertumbuhan dan Kadar Lipid Chlorella sp. Universitas Islam Negeri Maulana Malik Ibrahim. Malang.

Rosyadi., Agusnimar., Melati, H., 2019. Pemberian POC dengan Rentang Waktu Berbeda terhadap Kelimpahan Chlorella sp. Jurnal Dinamika Pertanian, 27(3): 171-178.

Septian, H., Hasan, H., Farida., 2017. Natural Feeding Artemia sp, Chlorella sp, and Tubifex sp to Growth and Survival Larvae Fish Comet (Carassius auratus). Jurnal Ruaya, 5(2): 21-27.

Sergejevova, M., Masojidek, J., 2012. Chlorella Biomass as Feed Supplement for Freshwater Fish: Sterlet, Acipenser ruthenus. Aquac. Res. 44: 157–159.

Sigalingging FA., Padil., Muria, S.R., 2019. Kultivasi Mikroalga Menggunakan Media Af6 berdasarkan Perbedaan Volume Solution A Media Af6. Jom FTEKNIK, 6(1): 1-5.

Sudhakar, K., Suresh, S., Premalatha, M., 2011. An Overview of CO2 Mitigation using Algae Cultivation Technology. International Journal of Chemical Research, 3(3): 110-117.

Susilaningsih, D., Lestari, S., Kusnadi., Hidayat, T., Susanti, H., 2014. Sago Waste Efficacy as Nutrition Rich Substrate for Microalgae LIPI11-2-AL002 Isolate. Berita Biologi, 13 (3): 301-307

Sylvester, B.D., Nelvy, D., Sudjiharno., 2002. Persyaratan Budidaya Fitoplankton. Budidaya Fitoplankton & Zooplankton. Balai Besar Pengembangan Budidaya Laut. Direktorat Jenderal Perikanan Budidaya. Departemen Kelautan dan Perikanan, 10:24-36.

Tamalonggehe, J., Kemer, K., Paransa, D.S.A.J., Mantiri, D.M.H., Kawung, N.J.,Undap, S.L., 2020. Efek Senyawa Timbal Asetat terhadap Pertumbuhan dan Kandungan Pigmen Klorofil Mikroalga Dunaliella sp. Jurnal Pesisir dan Laut Tropis, 8(2): 1-10.

Taradifa, S., Hasibuan, S., Syafriadiman., 2022. Pemanfaatan Pupuk Organik Cair Azolla sp. Terhadap Kepadatan Sel Chlorella sp. Jurnal Riset Akuakultur, 17(2): 85-93

Wibowo A.L., Mutiara D., Anggraini I.Y., 2017. Penggunaan Pupuk Organik Cair Hasil Fermentasi dari Azolla pinnata terhadap Kepadatan Sel Spirulina sp. Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan, 12(1): 56-65.

Wood, A.M., Everroad, R.C., Wingard, L.M., 2005. Measuring Growth Rates in Microalgal Cultures. Algal Culturing Techniques. pp 269-285. https://dx.doi.org/10.1016/B978-012088426-1/50019-6

Zahran, E., Risha, E., 2014. Modulatory Role of Dietary Chlorella vulgaris Powder against Arsenic-induced Immunotoxicity and Oxidative Stress in Nile Tilapia (Oreochromis niloticus). Fish Shellfish Immunol., 41: 654–66




DOI: http://dx.doi.org/10.31258/jnat.21.1.55-62

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