The Role of Kolkisin in Multiplication of Planlet Banana Kepok Abu Poliploidi in Vitro
Abstract
Bananas are one of the horticultural crops of the fruit group that have quite high social and economic value. One type of banana that is very popular is the pisang kepok. The main obstacle in pisang kepok production is the availability of seedlings. The above constraints can be overcome by propagation of banana seedlings using tissue culture techniques by adding colchicine to the tissue culture media. Colchicine is often used to improve plant quality. This study aims to determine the effect of adding colchicine to the media on the growth and formation of pisang kepok abu plantlet. The study was conducted from November 2019 - February 2020 at the MTC Laboratory, PT Great Giant Pineapple PG 4, East Lampung. The concentration of colchicine added to the media was 0.1%. The growth and development of plantlets produced from media with colchicine treatment will be compared with plantlets from the control group. The tests were repeated 10 times. The results showed that the growth and formation of pisang kepok abu plantlets from the treatment of colchicine were lower than the growth and formation of the pisang kepok plantlets in the control group.
Keywords
Full Text:
PDFReferences
Abdillah, F., 2010. Modifikasi Tepung Pisang Tanduk (Musa paradisiaca Formatypica) Melalui Proses Fermentasi Spontan dan Pemanasan Otoklaf untuk Meningkatkan Kadar Pati Resisten. Bogor (ID): Institut Pertanian Bogor.
Damayanti, F., Mariska, I., 2003. Induksi Poliploidi dengan Kolkisin pada Hibrid F1 hasil persilangan antar spesies pada tanaman panili asal ciamis. Berita Biologi, 06(04)
Dounias, E., 2008. Gloriosa superba L. Protologue Sp. Pl. 1 : 305 (1753).
Fajrina, A., Idris, M., Mansyurdin., Surya, N., 2012. Penggandaan Kromosom dan Pertumbuhan Somaklonal Andalas (Morus macroura Miq. Var macroura) yang Diperlakukan dengan Kolkhisin. Jurnal Biologi Universitas Andalas. 1(1)
Griesbach, R.J., 1985. Polyploidy in Phalaenopsis orchid improvement. The Journal of Heredity 76:74-75.
Haryanti, S., Hastuti, R.B., Setiari, N., Banowo, A., 2009. Pengaruh Kolkisin Terhadap Pertumbuhan, Ukuran Sel Metafase dan Kandungan Protein Biji Tanaman Kacang Hijau (Vigna radiata (L.) Wilczek). Jurnal Penelitian Sains & Teknologi, 10(2): 112 – 120.
Herman., Malaum I.N., Roslim, D.I., 2013. Pengaruh Mutagen Kolkisin Pada Biji kacang Hijau (Vigna radiata L.) Terhadap Jumlah Kromosom dan Pertumbuhan. J. BioETI. : 13-20.
Maghfirah., Rofiq, M., Nihayati, E., 2018. Pengaruh Pemberian Kolkisin Terhadap Keragaman Pertumbuhan Dua Klon Temulawak (Curcuma xathorriza Roxb.) Secara In Vitro. Jurnal Produksi Tanaman, 6(6): 1000 -1004.
Nurhasanah, E., 2017. Biodiversitas Plasma Nutfah Pisang (Musa spp.) Berdasarkan Jumlah Kromosom dan Tipe Genom di Kota Bandar Lampung. Skripsi. FMIPA. Universitas Lampung.
Rauf, S., Khan, I.A., Khan, F.A., 2006. Colchicine -induced tetraploidy and changes in allele frequencies in colchicine-treated populations of diploid assessed with RAPD markers in Gossypium arboreum L. Turkey Journal of Biology, 30:93-100.
Sulistianingsih, R., Suyanto Z.A., Anggia, N.E. 2004. Peningkatan Kualitas Anggrek Dendrobium Hibrida Dengan Pemberian Kolkhisin. J. Ilmu Pertanian. 11 (1): 13-21.
Wiendra, N.M.S., Pharmawati, M., Astuti, N.P.A., 2011. Pemberian Kolkhisin dengan Lama Perendaman Berbeda Pada Induksi Poliploidi Tanaman Pacar Air (Impatiens balasamina L.). Jurnal Biologi, XV(1): 9-14.
Yulianti, F., Purwito, A., Husni, A., Dinarti, D., 2015. Induksi tetraploid tunas pucuk jeruk siam simadu (Citrus nobilis Lour) menggunakan kolkisin secara in vitro. Jurnal Agronomi Indonesia, 43(1):66-71.
DOI: http://dx.doi.org/10.31258/jnat.18.2.76-81
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Dewi Sartika, Rochmah Agustrina, Eti Ernawiati, Bambang Irawan
This work is licensed under a Creative Commons Attribution 4.0 International License.