Amobilisasi Inulinase Aspergillus clavatus Gmn 11.3 Galur Lokal Indonesia dengan Matrik Karbon Aktif

Saryono Saryono, Henny Olivia R.M, Elvita Sepriana, Andi Dahliati, Chainulfiffah A.M

Abstract


Two types of inulinase are produced by Aspergillus clavatus Gmn 11.3 within the 3rd and 5th days of fermentation. The optimum condition of two types of immobilized inulinase is achieved using 20 grams of activated carbon, 200 meshes with protein adsorption of 96.71% and 96.19% respectively. Following immobilization of inulinase, incubation was carried out for 30 hours to hydrolyze inulin. After incubation, the proteins retained on the matrix are 66.96% of the 3 days fermentation enzymes and 37.36% for 5 days of fermentation enzyme.


Keywords


carbon active, immobilize, inulin, inulinase

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References


Alexander R.R., Griftith, J.M., & Wilkinson, M.L. 1985. Basic Biochemical Methods. Singapore: John Willey & Sons.

Amos. 1990. Amobilisassi enzim invertase dengan menggunakan karbon aktif. Skripsi S1. Pekanbaru: Jurusan Kimia FMIPA Universitas Riau.

Chibata, I. 1978. Immobilized Enzyme: research and development. Tokyo: Kodansha Ltd.

Ettalibi M. & Barrati, J.C. 1987. Purification, properties, and comparison of Invertase, exoinulinase, and endoinulinase of Aspergillus ficuum. J. Appl. Microbiology-Biotechnology 26(1): 13-20.

Mangunwidjaja, D., Suprihatin, & Sunarti, T.C. 1992. Pendayagunaan inulin dahlia (Dahlian pinnata) untuk ultrahigh fructose syirup dengan teknik imobilisasi sel Kluyveromyces marxianus dan enzim inulinase. Laporan Penelitian. Fakultas Teknologi Pertanian. Bogor: IPB.

Nakamura T., Nagatomo, Y., Hamada, S., Nisino, Y., & Ohta, K. 1994. Occurance of two form of extracellular endoinulinase from Aspergillus niger mutant 817. Journal of Fermentation and Biotechnology 78(2): 134-139.

Saryono, Soekartadireja, E.M., Supriatna, & Hadiman, H.R. 1999. Identifikasi jamur penghasil enzim inulinase pada rizosfir umbi dahlia (Dahlia variabilis). Kongres HITI VII. Bandung.

Saryono. 2002. Pemurnian dan penentuan beberapa sifat inulinase ekstraselular dari isolat jamur lokal yang tumbuh pada umbi dahlia (Dahlia variabilis). Disertasi Pasca Sarjana. Bandung: Universitas Padjajaran.

Saryono, Martina, A., & Chainulfifah. 2002. Isolasi dan karakterisasi jamur penghasil inulinase yang tumbuh pada umbi dahlia. Jurnal Natur Indonesia. 4(2): 171-177.

Vandamme, E.J. & Derycke, D.G. 1983. Microbial inulinases process, properties and applications. Adv. Appl. Microb.29: 139-176.

Winarno, F.G. 1983. Enzim Pangan. Jakarta: Penerbit PT Gramedia.

Zul, D. 1999. Produksi, purifikasi dan karakterisasi inulinase rizosfer Dahlia pinnata. Tesis Pasca Sarjana. Yogyakarta Program Studi Bioteknologi Jurusan Antar Bidang UGM




DOI: http://dx.doi.org/10.31258/jnat.10.1.31-35

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Copyright (c) 2008 Saryono Saryono, Henny Olivia R.M, Elvita Sepriana, Andi Dahliati, Chainulfiffah A.M

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