Respon Histopatologis Hepar Mencit (Mus musculus) yang Diinduksi Benzo(α)Piren terhadap Pemberian Taurin dan Ekstrak Daun Sirsak (Annona muricata)

Annisa Agata, Endang Linirin Widiastuti, G. Nugroho Susanto, Sutyarso '

Abstract


Cancer is a disease that is characterized by the existence of damage and cell abnormality in growth and differentiation. Liver cancer is a disorder of hepar tissue derivated from its tumors. Taurine is known as antioxidant but its role as anticancer needs to be explored more as well the role of Annona muricata leaf extract which is believed to have its role as anticancer substance. This research, therefore, aimed to explore the effect of taurine and Annona muricata leaf extract on the hepar histopathology of male mice (Mus musculus) induced by benzo(α)pyren in vivo. This research was carried out by using a complete randomized design, which consisted of 5 treatment groups which was repeated 5 times. Group I was given 0.2 mL corn oil for 15 days, group II was induced by benzo(α)pyren without taurin nor A. Muricata leaf extract for 10 days, group III was given 7.8 mg taurine/BW/day (twice a day) starting from the 15 th days before the induction of benzo(α)pyren, group IV, after induced with benzo(α)pyren, taurine was given with dosage of 7.8 mg/BW/day, group V, after induced with benzo(α)pyren, soursop leaf extract was given with amount of 277.8 mg/BW/day. Data analyzed by Kruskal-Wallis test and one way ANOVA with Fisher test (p>0.05). The results indicated that taurine had ability to recover the liver tissue induced by benzo(α)pyren as (carcinogenic) while, Annona muricata leaf extract had not shown any recover of tissue damage

Keywords


benzo(α)phyren, hepar, leaf soursop (Annona muricata), taurine

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References


Akhila, J,S., Shyamjith, Deepa &Alwar, M.C. 2007. Acute toxicity studies and determination of median lethal dose. Curr Sci 93(7): 917-920.

Amelia, F,E., Angeline, K & Wahyu. 2012. Tablet salut enteric ekstrak etanol daun sirsak (Annona muricata) sebagai antikanker kolon yang potensial. Skripsi Sarjana. Yogyakarta: Universitas Gadjah Mada.

Ammer, E,M., Shaaban, A.A., Ghonem, H.A & Elkashef, H.A. 2013. Effect of Taurine on The Respiratory System of Rats. J Food Pharm Sci 1(2): 22 29.

Baroroh, H.N & Warsinah. 2009.Antiinflammatory activity of ethanolic extract of leaves of jarak pagar (Jatropa curcas L.) and neutrophils profile in rats foot induced Carrageenan. Proceedings of International Conference On Medicinal Plants. Surabaya, 22–23.

Fajariyah, S., Utami, E.T & Arisandi, Y. 2010. Efek pemberian estrogen sintetis (Diethylstillbestrol) terhadap struktur hepar dan kadar SGOT dan SGPT pada mencit (Mus musculus) betina strain Balb/C. J Ilmu dasar 11(1): 76–82.

Greaves, M.B.P., Ch, B & Path, F.R.C. 2000. Histopathology of Preclinical Toxicity Studies. Amsterdam: Elsevier.

Hanahan, D & Weinberg, R.A. 2000. The hallmarks of cancer. Cell 100(2): 57–70.

Hariyatmi. 2004. Kemampuan Vitamin E sebagai Antioksidan terhadap Radikal Bebas pada Usia Lanjut. MIPA 14(1): 52–60.

Katz, B.G. 2002. Basic and clinical pharmacology, alih bahasa : Dripa Sjabana, Endang Isbianti, Achmad Basori, Moch, Sudjak N, Indriyatni, Ramadhani RB, Sunarni Zakaria, Salemba Medika, Surabaya.

King, R.J.B. 2000. Cancer Biology, 2nd ed,. Pearson Education Limited. London.

Klaassen, C.D. (ed). 2001. Casarett and Doull’s Toxicology. The Science of Poison. McGraw Hill. New York.

Li, N., Shi, Z., Tang, Y., Chen, J & Li, X. 2008. Recent progress on the total synthesis of acetogenins from Annonaceae. Beilstein Journal of Organic Chemistry 4(48): 4–12.

Ma’at, S. 2004. Obat tradisional untuk pelayanan kesehatan formal. Prosiding Seminar Nasional. Surabaya. 5 September 2004.

Niendya, W.A., Muhammad, A.D & Teguh, S. 2011. Rasio Hepar Bobot-Tubuh Mencit (Mus musculus) setelah Pemberian Diazepam, Formalin, dan Minuman Beralkohol. Buletin Anatomi dan Fisiologi XI(1) Maret 2011.

Ngabekti, S & Isnaeni, W. 2000. Pemanfaatan Kurkumin Untuk Mengeliminir Pengaruh Diazonin terhadap Kerusakan Hati Mencit (Mus musculus L). Semarang: Fakultas MIPA Universitas Negeri Semarang.

Prasetya, G & Laksono, H. 2013. Ekstrak daun sirsak (Annona muricata) menggunakan pelarut etanol. Jurnal Tekonologi Kimia Industri 1(2): 111–115.

Ren, W., Qiao, Z., Wang, H., Zhu, L & Zhang, L. 2003. Flavonoids: promising anticancer agent. Medicinal Research Review 23(4): 519–534.

Retnani, V. 2011. Pengaruh suplementasi ekstrak daun Annona muricata terhadap kejadian displasia epitel kelenjar payudara tikus sprague dawley yang diinduksi 7,12-dimetilbenz(Q)antrasena (DMBA). Skripsi Sarjana. Semarang: Fakultas Kedokteran Universitas Diponegoro.

Smayda, R. 2002. Contemporary review of therapeutic benefits of the amino acid taurin. The Journal of Biological Chemistry 257(6): 2802–2805.

Schaffer, S., Ramila, K.C., Jong, C.J., Ito, T & Azuma, J. 2009. Role of protein phosphorylation in Tau TKO cardiomyopathy, Int Taurine Symp.

Sukardja, IDE. 2000. Onkologi Klinik edisi 2. Surabaya: Airlangga University Press.

Sreelatha, S & Padma, P.R. 2009. Antioxidant activity and total phenolic content of Moringa oleifera leaves in two stages of maturity. Plant foods hum nutr 64(4): 302–311.

Sumpena & Yana. 2009. Uji Mutagenisitas Benzo (alfa) piren dengan Metode Mikronukleus pada Sumsum Tulang Mencit Albino (Mus musculus). Cermin Dunia Kedokteran 36(1): 33–36.

Syahrizal, D. 2008. Pengaruh Proteksi Vitamin C terhadap Enzim Transaminase dan Gambaran Histopatologis hati Mencit yang Dipapar Plumbum. Tesis Pasca Sarjana. Medan: Fakultas Kedokteran. Universitas Sumatera Utara.

Uche, F.I & Aprioku, J.S. 2008. The phytochemical constituents, analgesic and antiinflammatory effects of methanol extract of jatropha curcas leaves in mice and wister albino rats. J Appl Sci Environ Manage 12(4): 99–102.

Waji, R,A & Sugrami, A. 2009. Makalah kimia organik bahan alam flavonoid (quercetin). Makassar: Universitas Hasanuddin 8–9.




DOI: http://dx.doi.org/10.31258/jnat.16.2.54-63

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