Enzyme–Catalysed Synthesis of Palm-Based Wax Esters-A Kinetic Study
Abstract
Wax esters are long chain esters that are derived from fatty acids and alcohols with chain lengths of 12 carbons or more. Wax esters havea wide range of application in industrial especially in cosmetics. The present work focuses on the synthesis of wax esters by alcoholysisreaction from palm oil and oleyl alcohol in hexane by lipase from Rhizomucor meihei (Lipozyme IM). Effect of various concentrations ofpalm oil and oleyl alcohol were studied to deduce the kinetics and mechanism of the reaction. The alcoholysis reaction followed Michaelis-Menten kinetics. The reaction follows a Ping-pong Bi-Bi mechanism. The maximum rate was estimated to be 6 x 10 -3 mmol/h. mg catalystand the Michaelis-Menten constant for palm oil and oleyl alcohol were 4.145 M and 6.120 M, respectively.
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Awang, R., Basri, M., Ahmad, S. & Salleh, A.B. 2004. Lipase- catalyzed esterification of palm-based 9,10-dihydroxystearic acid and l-octanol in hexane- a Kinetic study. Biotechnology Letters 26: 11-14.
Chen, J.P. & Wang, J.B. 1997. Wax ester synthesis by lipase- catalyzed esterification with fungal cells immobilized on cellulose biomass support particles. Enzyme and Microbial Technology 18: 615-622.
Garcia, T., Sanchez, N., Martinez, M. & Aracil, J. 1999. Enzymatic synthesis of fatty esters part II, Optimization studies, Enzyme and Microbial Technology 25: 591-597
George, S., Kichhorff, R. & Mukherjee, K.D. 2000. Lipase catalyzed alcoholysis of crambe oil and camelina oil for The preparation of long-chain esters, Journal of American oilChemistry Society 61(2): 191-195
Gunawan, E.R. & Suhendra, D. 2008a. Four-factor response surface optimization of the enzymatic synthesis of wax ester from palm kernel oil. Indonesian Journal of Chemistry 8(1): 83-90
Gunawan, E.R. & Suhendra, D. 2008b. Wax ester production by alcoholysis of palm oil fractions. Indonesian Journal of Chemistry 8(3): 356-362.
Kline, S. 1956. French international co, improvements in or relating to pharmaceutical tablet or pellet and method of preparing the same, GB 747914.
Knox, T. & Cliffe, K.R. 1984. Synthesis of long-chain esters in a loop reactor system using a fungal cell bound enzyme, Process Biochemistry 19: 188-192.
Lai, D.T., Hattori, N. & O’connor, J. 1999. Kinetics of enzymatic synthesis of isopropylidene glycerol ester by goat pregastric lipase, Journal of American Oil Chemists’ Society 76(7): 845- 851.
Mat Radzi, S., Basri, M., Salleh, A.B. & Abdurrahman, M.B. 2005. Large scale production of liquid wax ester by immobilized lipase, Journal of Oleo Science 54: 203-209.
Peter, T.R. & Robert, B. 2001. Beeswax through the ages.Personal Care. Oct 27-31.
PORIM Technology 16. 1986. Malaysian palm kernel stearin. Palm Kernel Olein and Their hydrogenated products. Bangi: Palm Oil Research Institute Malaysia. 1-9.
Roberts, D.V. 1977. Enzyme Kinetics, London: Cambridge University Press.
Salis, A., Solinas, V. & Monduruzzi, M. 2003. Wax ester synthesis from heavy fraction of sheep milk fat and cetyl alcohol by immobilised lipases. Journal of Molecular Catalysis. B: Enzymatic 21: 167-174.
Sanchez, N., Martinez, M.J., Aracil & Corma, A. 1992. Synthesis of oleyl oleate as jojoba oil analog. Journal of American Oil Chemists’ Society 69: 1150-1153.
Yadav, G.D. & Lathi, P.S. 2003. Kinetics and mechanism of synthesis of butyl isobutyrate over immobilised lipases. Biochemistry Engineering Journal 16: 245-252
Zaidi, A., Gainer, J.L., Carta, G. & Mrani, A. 2002. Esterification of fatty acids using nylon-immobilized lipase in n-hexane: kinetic parameters and chain length effect. Journal of Biotechnology 93: 209-216.
DOI: http://dx.doi.org/10.31258/jnat.14.1.37-41
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