Genetic Diversity and The Relationship Between The Indonesian Mangosteen (Garcinia Mangostana) and The Related Species Using Isozyme Markers

Soaloon Sinaga, Sobir Sobir, Roedhy Poerwanto, Hajrial Aswidinnoor, Dedy Duryadi

Abstract


Indonesia was known to have high diversity of mangosteens (Garcinia mangostana) and the related species. Inorder to elucidate the genetics variability of the diversity, thirty three accessions were examined by using isozymeanalysis. The genetic diversity and relationships among several mangosteens and other Garcinia sp wereestablished by using four isozymes. The level of polymorphism as revealed by isoenzyme was 88%. Althoughmangosteen is believed to reproduce exclusively through apomixis, our result show that considerable geneticdiversity exists within G. mangostana and between other Garcinia species. Based on 27 bands there were 5-42%dissimilarity level among mangosteen accessions, while the other species has 75% dissimilarity. The dendrogramis built based on isozyme marker analysis to separate clusters of mangosteen and other Garcinia sp. The datashowed that G. mangostana is a close relative of G. malaccensis, G. porrecta, G. celebica, and G. hombroniana. Theconcurrence analysis on isozyme analysis result showed the very good fit of Rolf correlation value (0.914). Thisresult indicated that isozymes could group G.mangostana and the related species.

Keywords


genetic diversity, isozymes, mangosteens, related species

Full Text:

PDF

References


Drew, R.A. 1997. The Application of Biotechnology to the Conservation and Improvement of Tropical and Subtropical Fruit Species. Seed and Plant Genetic Resources Service. Rome: Food and Agriculture Organization of the United Nations.

Ford, H. & Richards, A.J. 1985. Isozyme variation within and between Taraxacum agamospesies in a single locality. Heredity 55: 289-291.

Hughes, J. & Richards, A.J. 1985. Isozyme in heritance in diploid Taraxacum hybrid. Heredity 54: 245-249.

Mansyah, E., Edison, H.S. & Winarno, M. 1994. Eksplorasi dan Studi Keragaman Manggis (Garcinia mangostana L.) di Sumatera Selatan. Laporan Hasil Penelitian Balai Penelitian Hortikultura Solok.

Mansyah, E., Jawal, M., Lukitariati, A. & Susiloadi, A. 1999. Variabilitas genetik tanaman manggis melalui analisis isoenzim dan kaitannya dengan variabilitas fenotipik. Zuriat 10(1): 1-9.

Nakonechnaya, O.V., Koren, O.G. & Zhuravlev, Yu. N. 2007. Allozyme variation of the relict plant Aristolochia manshuriensis Kom. (Aristolochiaceae). Russian Journal of Genetics 43(2): 156-164.

Nei, M. & Li, W.H . 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl acad Sci USA 76(10): 5269-5273.

Osman, M. & Abdul, R.M. 2006. Mangosteen Garcinia mangostana L. Southampton Center for Underutilised Crops, University of Southampton, UK. 169p.

Ramage, C.M., Sando, L., Peace, C.P., Carroll, B.J. & Dew, R.A. 2004. Genetic diversity revealed in the apomictic fruit species Garcinia mangostana L. (mangosteen). Euphytica 136(1): 1-10.

Richards, A.J. 1990. Studies in Garcinia, dioecious tropical forest trees: the origin of the mangosteen (G. mangostana). Botanical Journal of The Linn.Soc 103:103-308.

Rolf, F.J. 1998. NTSys-pc. Numerical Taxonomy and Multivariate Analysis Syatem. Version 2.02. Exerter Software. New York.

Soltis, D.E. & Soltis, P.S. 1989. Isoenzimes in Plant Biology. Portland, Oregon: Discorides Press.

Supriyanto, A., Muharam A. & Hariyanto, B. 1999. Evaluasi keragaman pohon manggis pada sentra produksi di Jawa dan Lombok dengan analisis isoenzim. Bul. Plasma Nutfah 5(1): 6-10.

Thangaraja, A. & Ganesan V. 2007. Allozyme diversity in South Western Ghats populations of Terminalia paniculata Roth. (Combretaceae). African Journal of Plant Science. 1(1): 7-13.




DOI: http://dx.doi.org/10.31258/jnat.13.1.53-58

Refbacks

  • There are currently no refbacks.


Copyright (c) 2012 Soaloon Sinaga, Sobir, Roedhy Poerwanto, Hajrial Aswidinnoor, Dedy Duryadi

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.