Failed to fetch OJS Article Content. Penghambatan Ekstrak Etanol Sereh (Cymbopogon citratus (DC) Stapf ) terhadap Produksi Verotoksin Escherichia coli Verotoksigenik | Cepeda | Jurnal Natur Indonesia

Penghambatan Ekstrak Etanol Sereh (Cymbopogon citratus (DC) Stapf ) terhadap Produksi Verotoksin Escherichia coli Verotoksigenik

Gino Nemesio Cepeda, Ratih Dewanti Hariyadi, Supar Supar

Abstract


Citronella was a spicy plant used as seasoning in Indonesian food. It was reported to have antimicrobial activity. Itsextract in form of volatile oil prepared by distilation can inhibit the growth of some bacteria. The objective of theresearch was to know the inhibition of ethanolic extract of the citronella on growth and verotoxin production ofverotoxigenic Escherichia coli. The assay of antimicrobial activity was done by using broth dilution and verocellassay. The results indicated that minimum inhibitory concentration of extract can inhibit effectively verotoxinproduction of verotoxigenic Escherichia coli.

Keywords


antimicrobial, citronella, extract, verotoxigenic Escherichia coli

Full Text:

PDF

References


Adams, M.R. & Moss, M.O. 1995. Food Microbiology. Cambridge: The Royal Society of Chemistry.

Anonim. 2007. Ekstrak senyawa aktif buah picung sebagai penghambat bakteri pembusuk dan penghasil histamin. http:/ /rr-1.blog spot.com/2007/08. picng-as-naturan-preervative.html.(10 Agustus 2009).

ATTC, (American Type Culture Collection). 1989. Catalogue of Bacteria & Bacteriophages. 17th ed. USA:Rockville Maryland.

Bloomfield, S.F. 1991. Methods for assessing antimicrobial Activity. Di dalam: Denyer S P, Hugo WB, (ed). Mechanism of Action of Chemical Biocides Their Study and Exploitation. London : Blackwell scientific Publication.

Carson, C.F. & Riley, T.V. 1995. Antimicrobial activity of the major components of essential oil of Melaleuca aternifolia. J. Appl. Bacteriol 78:264.

Cepeda, G.N. 2009. Penghambatan ekstrak etanol sereh (Cymbopogon citratus (DC.) Stapf) pada beberapa konsentrasi garam dan pH terhadap pertumbuhan Escherichia coli verotoksigenik. J. Agrotek 1(4): 9-17.

Duraipandiyan, V., Ayyanar, M. & Ignacimuthu, S. 2006. Antimicrobial activity of some ethnomedical plants used by Paliy tribe from Tamil Nadu India. BMC Complementary and Alternative Med 6:35.

Fardiaz, S., Triana, A. & Rahayu, W.P. 1998. Aktivitas antimikroba bumbu segar hasil olahan industri terhadap bakteri patogen dan perusak makanan. J. Ilmu dan Tek. Pangan 3(2): 1-9.

Frazier, W.C. & Westhof, D.C. 1979. Food microbiology. New Delhi : Tata McGraw Hill Publishing Co. Ltd.

Friedman, M. 1997. Chemistry, biochemistry and dietary role of potato polyphenols. Rev. J. Agric. Food Chem. 45: 1523-1540.

Friedman, M., Henika, P.R. & Mandrell, R.E. 2002. Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes and Salmonella enterica. J. Food Prot 65: 1545-1560.

Hammer, K.A., Carson, C.F. & Riley, T.V. 1999. Antimicrobial activity of essential oils and other plants extract. J. Appl. Microbiol 86: 985-990.

Hostetmann, K., Wolfender, J.L. & Rodrigue. 1977. Rapid detection and subsequent isolation of bioactive constituents of crude plant extracts. Planta Med 63: 2-10.

Jay, J.M. 2000. Modern food microbiology. Maryland: Aspen Publishing Inc.

Jenie, B.S.L., Undriyani,K. & Dewanti, R. 1992. Pengaruh konsentrasi jahe dan waktu kontak terhadap aktivitas beberapa mikroba penyebab kerusakan pangan. Bul. Pen. Ilmu dan Tek Pangan III 2: 1-16.

Kusmiyati. & Supar. 1998. Escherichia verotoksigenik dari anak sapi perah penderita diare. Prosiding seminar hasil-hasil penelitian Bogor. Balai Penelitian Veteriner, Penelitian dan Pengembangan Peternakan, Badan Penelitian dan Pengembagan Pertanian Departemen Pertanian Bogor. Bogor, 18-19 Februari 1998.

Lusiastuti, A.M. 1994. Pengaruh Klorin terhadap Daya Hidup dan profil resistensi antibiotika bakteri fekal koliform dan Escherichia coli O157H7 dari limbah cair rumah potong ayam. Program Studi Kedokteran Hewan. Bogor: Institut Pertanian Bogor.

Marques, L.R.M., Moore, M.A., Wells, J.G., Wachsmuth, L.K. & O’Brien, A.D. 1989. Production of shiga-like toxin by Escherichia coli. J. Infect. Dis 154: 338-341.

Oyofo, B.A. et al., 2002. Entheropatogens associated with acute diarrhea in community and hospital patients in Jakarta, Indonesia. FEMS Immun. Med. Microbiol 34: 139-146.

Palumbo, S.A., Call, J.E., Schultz, F.J. & Williams, A.C. 1995. Minimum and maximum temperatures for growth and verotoxin production by hemorrhagic strains of Escherichia coli. J. Food Prot. 58: 352-356.

Pramono, F. 2000. Evaluasi Media Pengkayaan Selektif untuk mendeteksi Escherichia coli O157:H7 dalam daging giling lokal. Fakultas Teknologi Pertanian. Bogor: Institut Pertanian Bogor.

Ray, B. 2001. Fundamental food microbiology. 2nd ed. New York: CRC Press.

Rhon S., Rawel, H.M. & Kroll, J. 2002. Inhibitory effects of plant phenols on the activity of selected enzymes. J. Agric. Food Chem 50: 3566-3571.




DOI: http://dx.doi.org/10.31258/jnat.13.1.72-76

Refbacks

  • There are currently no refbacks.


Copyright (c) 2012 Gino Nemesio Cepeda, Ratih Dewanti Hariyadi, Supar

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.