Efek Beta Karoten dan Agregasi Klorofil Pada Fotostabilitas Klorofil a dalam Pelarut Aseton

Junet. F. da Costa, Ferry F Karwur, Leenawaty Limantara


Chlorophyll as photosynthetic pigment has many benefits to human such as antioxidant, antibacterial, color additive,immunity, and photosensitizer. Concerning to these applications, chlorophyll a will react with oxygen and light andreduce its effectiveness. It is then important to understand the stability of chlorophyll a on oxygen and light.Stability of chlorophyll a in the presence of beta-carotene (1:1) in acetone was studied for various waterconcentrations. Each solution was exposed to red light (ë e” 630 nm) for 0.5; 1.0; 2.5; 5.0; 10; 20; 30; 40; 50; and 60minutes and their spectrum were analysed. When water was added (8.33; 16.67; 25; 33.33; 41.67; 50; 58.33; 66.67;75; and 83.33 percent) to the acetone solution of chlorophyll a, the chlorophylls aggregate, and in the presence ofbeta-carotene, the chlorophyll more stable. The water shifts the chlorophyll a spectrum toward red duringillumination with or without the presence of carotene. The formation of oligomeric chlorophyll a aggregate occurswithin 10-20 minutes after the water was applied. This strategy of aggregating the chlorophylls to dissipateexcess energy captured from light does not support the photostability of chlorophyll as much as beta-carotene.Surprisingly, when beta carotene was mixed with 66.7% and 75.0% of water, the chlorophyll a degraded 5.56% and9.71% respectively. In conclusion, the aggregate form of chlorophyll a and the presence of beta-carotene increasephotostability of chlorophyll a in acetone solution.


aggregation, beta carotene, chlorophyll a, photostability.

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Abeliovich, A. & Shilo, M. 1972. Photoxidative death in blue- green algae. J. Bacteriol 111: 682-689.

Agostiano, A., Catucci, L. Cosma, P. & Fini, P. 2003. Aggregation processes and photophysical properties of chlorophyll a in aqueous solution modulated by presence of cyclodextrins. Phys. Chem. 5: 2122-2128.

Anderson, A. F. H., & Calvin, M. 1964. The aggregation of chlorophyll a. Archives of biochemistry and biophysics 107: 251-259.

Barzda, V., Peterman, E. J. G., Van Grondelle, R. & Van Amerongen, H. 1998. The Influence of aggregation on triplet formation in light-harvesting chlorophyll a /b pigment-protein complex II of green plants. Biochem 37: 546-551.

Brandis, A. S, Salomon, Y. & Scherz, A. 2006. Chlorophyll sensitizer in photodynamic therapy. Di dalam: Grimm, B., Porra, R. Rüdiger, W. & Scheer, H (ed). Chlorophyll and Bacteriochlorophyll: Biochemistry, Biophysic, Function and Apllications. Netherlands: Springer.

Britton, G., Liaaen-Jensen, S. & Pfander, H. 1995. Carotenoids Today and Challenges for The Future. Di dalam: Britton, G. Liaaen-Jensen, S. & Pfander, H. (ed). Isolation and Analysis. Vol.IA. Birkhäuser Verlag Basel.

da Costa, J. F., Karwur, F. F. & Limantara, L. 2007. Fotoproteksi Beta Karoten dan Lutein Terhadap Klorofil a dalam Aseton. Prosiding Seminar Pigmen Nasional 2007 Back To Nature Dengan Pigmen Alami. Salatiga, 24 Agustus 2007.

de Paula, J. C., Robblee, J. H. & Pasternackt, R. F. 1995. Aggregation of chlorophyll a probed byresonance light scattering spectroscopy. Biophys. J. 68: 335-341

Fiedor, J., Fiedor, L. Winkler, J. Scherz, A. & Scheer, H. 2001. Photodynamic of the Bacteriochlorophyll-Carotenoid System 1. Bacteriochlorophyll-photosensitized oxygenation of Beta-Caroten in Aceton. Photochem. Photobiol 74: 64- 71.

Fiedor, J, Fiedor, L. Kammhuber, N. Scherz, A. & Scheer, H. 2002. Photodynamics of the bacteriochlorophyll-carotenoid System. 2. Influence of central metal, solvent and beta carotene on photobleaching of bacteriochlorophyll derivatives. Photochem Photobiol 76: 145-152.

Gross, J. 1987. Pigments in Fruits. London: Kluwer Academic Publisher.

Gross, J.1991. Pigments in Vegetables: Chlorophyll and Carotenoids. New York: Van Nostrand Reinhold

Goedheer, J.C.1966. Visible Absorption and Flourescence of Chlorophyll and Its Aggregates in Solution. Di dalam: Vernon, L.P. & Seely, G. R. A. (ed). The Chlorophyll. New York: Academic Press.

Jeffrey, S. W, Mantoura, R. F. C. & Wright, S. W. 1997. Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods. Paris: UNESCO

Katz, J.J., Shipman, L. L. Cotton, T.M. & Janson, T.R. 1978a. Chlorophyll aggregation: Coordination interactions in chlorophyll monomers, dimers and oligomers. Di dalam: Dolphin, D. (ed). The Porphyrins. New York: Academic Press.

Katz, J.J, Norris, J. R. Shipman, L.L. Thurnauer, M. C. & Wasielewski, M. R. 1978b. Chlorophyll function in the photosyntetic reaction center. Ann. Rev. Biophys. Bioeng 7:393-434

Lichtenthaler, K. H. 1987. Methods in Enzymology. Chlorophyll and Carotenoid: Pigments of Photosynthetic Biomembranes. New York: Academic Press.Inc.

Porra, R. J., Pfündel, E. E. & Engel, N. 1997. Metabolism and function of photosyntesis. Di dalam: Jeffrey, S. W. Mantoura, R. F. C. & Wright, S.W. (ed). Phytoplankton Pigments in Oceanography. France: UNESCO.

Scheer, H., 1991. Chlorophylls. USA: CRC Press, Inc.

Scheer, H. 2006. An overview of chlorophylls and bacteriochlorophylls: biochemistry, biophysics, function and apllications. Di dalam: Grimm, B., Porra, R. Rüdiger, W. & Scheer, H (ed). Chlorophyll and Bacteriochlorophyll: Biochemistry, Biophysics, Function and Apllications. Netherlands: Springer.

Standfuss, J., Van Scheltinga, A. C. T. Lamborghini, M. & Kühlbrandt, W. 2005. Mechanisms of Photoprotection and Nonphotochemical Quenching in Pea Light harvesting Complex at 2.5Å resolution. The EMBO journal 24:919-928.

Vladkova, R., 2000. Chlorophyll a Self-assembly in Polar Solvent- Water Mixtures. Photochem Photobiol 71: 71-83

Wang, Y., Mao, L. & Hu, X. 2004. Insight into the structural role of carotenoids in photosystem I: A quantum chemical analysis. Biophys J. 86: 3097-3111.

Zurdo, J., Fernández-Cabrera, C. & Ramírez, J.M. 1992. Enhancement of carotenoid-to-chlorophyll singlet energy transfer by carotenoid-carotenoid interaction. Biophys. J. 61: 1462-1469.

DOI: http://dx.doi.org/10.31258/jnat.11.2.115-123


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